LOCUS       pSTVH8_N-BirA_pD       10574 bp    DNA     circular     29-AUG-2018
DEFINITION  .
ACCESSION   urn.local...5v-9dyp81f
VERSION
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com Cat.
            No. 632158,632162,632157,632163.
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label="5'\LTR"
     misc_binding    636..653
                     /label="PBS"
     misc_feature    685..822
                     /label="Packaging\signal"
     misc_binding    1303..1536
                     /label="RRE"
     misc_feature    2028..2151
                     /label="cPPT/CTS"
     attR2           complement(2459..2583)
                     /created_by="User"
                     /modified_by="User"
                     /label="ATTR2"
     CDS             complement(2624..2929)
                     /ApEinfo_fwdcolor="#00dd14"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="ccdB"
     CDS             complement(3271..3930)
                     /ApEinfo_fwdcolor="#00dd14"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Chlorm"
     attR1           complement(4039..4163)
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="ATTR1"
     CDS             complement(4176..4199)
                     /ApEinfo_fwdcolor="#006ddd"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="FLAG"
     CDS             complement(4230..5192)
                     /ApEinfo_fwdcolor="#2474dd"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BirA"
     promoter        complement(5250..5565)
                     /created_by="User"
                     /label="TRE"
     promoter        5610..5836
                     /created_by="User"
                     /label="EF1a-core"
     CDS             5853..6599
                     /ApEinfo_fwdcolor="#ff9562"
                     /ApEinfo_revcolor="#ff8040"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="rtTA"
     WPRE            6620..7195
                     /ApEinfo_fwdcolor="#008cff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="WPRE"
     LTR             7290..7926
                     /label="3'\LTR"
     rep_origin      complement(8395..9068)
                     /label="pUC\origin"
     CDS             complement(9213..10209)
                     /label="Amp(R)"
ORIGIN      
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtg catgcctgct
     2221 attgtcttcc caatcctccc ccttgctgtc ctgccccacc ccacccccca gaatagaatg
     2281 acacctactc agacaatgcg atgcaatttc ctcattttat taggaaagga cagtgggagt
     2341 ggcaccttcc agggtcaagg aaggcacggg ggaggggcaa acaacagatg gctggcaact
     2401 agaaggcaca gtcgaggctg atcagcgggt ttaaacgggc cctctagact cgagtcacac
     2461 cactttgtac aagaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     2521 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     2581 atggtcgacc tgcagactgg ctgtgtataa gggagcctga catttatatt ccccagaaca
     2641 tcaggttaat ggcgtttttg atgtcatttt cgcggtggct gagatcagcc acttcttccc
     2701 cgataacgga gaccggcaca ctggccatat cggtggtcat catgcgccag ctttcatccc
     2761 cgatatgcac caccgggtaa agttcacggg agactttatc tgacagcaga cgtgcactgg
     2821 ccagggggat caccatccgt cgcccgggcg tgtcaataat atcactctgt acatccacaa
     2881 acagacgata acggctctct cttttatagg tgtaaacctt aaactgcatt tcaccagccc
     2941 ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc gggcgacctc agccatccct
     3001 tcctgatttt ccgctttcca gcgttcggca cgcagacgac gggcttcatt ctgcatggtt
     3061 gtgcttacca gaccggagat attgacatca tatatgcctt gagcaactga tagctgtcgc
     3121 tgtcaactgt cactgtaata cgctgcttca tagcatacct ctttttgaca tacttcgggt
     3181 atacatatca gtatatattc ttataccgca aaaatcagcg cgcaaatacg catactgtta
     3241 tctggctttt agtaagccgg atccagatct ttacgccccg ccctgccact catcgcagta
     3301 ctgttgtaat tcattaagca ttctgccgac atggaagcca tcacaaacgg catgatgaac
     3361 ctgaatcgcc agcggcatca gcaccttgtc gccttgcgta taatatttgc ccatggtgaa
     3421 aacgggggcg aagaagttgt ccatattggc cacgtttaaa tcaaaactgg tgaaactcac
     3481 ccagggattg gctgagacga aaaacatatt ctcaataaac cctttaggga aataggccag
     3541 gttttcaccg taacacgcca catcttgcga atatatgtgt agaaactgcc ggaaatcgtc
     3601 gtggtattca ctccagagcg atgaaaacgt ttcagtttgc tcatggaaaa cggtgtaaca
     3661 agggtgaaca ctatcccata tcaccagctc accgtctttc attgccatac ggaattccgg
     3721 atgagcattc atcaggcggg caagaatgtg aataaaggcc ggataaaact tgtgcttatt
     3781 tttctttacg gtctttaaaa aggccgtaat atccagctga acggtctggt tataggtaca
     3841 ttgagcaact gactgaaatg cctcaaaatg ttctttacga tgccattggg atatatcaac
     3901 ggtggtatat ccagtgattt ttttctccat tttagcttcc ttagctcctg aaaatctcga
     3961 cggatcctaa ctcaaaatcc acacattata cgagccggaa gcataaagtg taaagcctgg
     4021 ggtgcctaat gcggccgcca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     4081 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     4141 tcagcttttt tgtacaaact tgtgggcgcg cctagtttat cgtcatcgtc tttgtagtct
     4201 ggcgcgccgc cgccggggcc agatcccccc ttctcggcgc tccgcaggct gatctcgccg
     4261 cccatccagg gcttgatgat gccgtcctgc tccagcagca gggcgccctg cttgtcgatg
     4321 ccccggctga tgccgaagat ctccttgtcg ccgatgatca gcttcacggg ccggttgatg
     4381 aagttgtcca gcttctccca ccggctcagg tagggggcca ggccctcctg ctcgaacagc
     4441 tccagggcgg cccgcagctc ccggatcagc atggcggcca gggtgttccg gtccaggttg
     4501 atgccggcct cctgcagggt gatccagccc tggttcacca cgctctcctc cacccgccgc
     4561 atggccatgt tgatgccggc gccgatcacg atctgggcgg cgtcgccggt cttgccggtc
     4621 agctccacca ggatgccggc cagcttccgg tcctgcaggt acaggtcgtt gggccacttc
     4681 acccgcacct tgtcggcgcc cagcttccgc agcacctcgg ccatcacgat gccgatcacc
     4741 aggctcaggc cgatggcggc ggcggggccc tgctccagcc gccagaacat gctcaggtac
     4801 aggttggcgc cgaaggggct gaaccacttc cggccccggc cgccccggcc ggcctgctgg
     4861 tactcggcga tgcaggcgtc gccgctcttc agctcgccga tccggtccag caggtactgg
     4921 ttggtgctgt cgatcacggg cagcacggcc acgctgccgc cgtccagctg gcccaggatc
     4981 tgcttggcgt tcagcagctg gatgggctcg ggcaggctgt agcccttgcc gggcacggtg
     5041 aacacgtcca cgccccagtc ccgcagggtc tggatgtgct tgttgatggc ggcccggctc
     5101 atgcccaggg tctcgcccag ctgctcgccg ctgtggaact cgccgttggc cagcagggcg
     5161 atcagcttca ggggcacggt gttgtccttc atggtggcgc tagctggatc cgagctcggt
     5221 accaagctta ggccgcggat ccttctccag gcgatctgac ggttcactaa acgagctctg
     5281 cttatatagg cctcccaccg tacacgccta cctcgacata cgttctctat cactgatagg
     5341 gagtaaactc gacatacgtt ctctatcact gatagggata aactcgacat acgttctcta
     5401 tcactgatag ggagtaaact cgacatacgt tctctatcac tgatagggag taaactcgac
     5461 atacgttctc tatcactgat agggagtaaa ctcgacatcg ttctctatca ctgataggga
     5521 gtaaactcga catacgttct ctatcactga tagggagtaa actctagtga agacgaaagg
     5581 gccagacgcg gccgctcaac gcgtgaattc ggtgcccgtc agtgggcaga gcgcacatcg
     5641 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     5701 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     5761 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     5821 gccgccagaa cacaggaact gcagcgctta ccatgtctag actggacaag agcaaagtca
     5881 taaacggcgc tctggaatta ctcaatggag tcggtatcga aggcctgacg acaaggaaac
     5941 tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag aacaagcggg
     6001 ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac ttctgccccc
     6061 tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcattc cgctgtgctc
     6121 tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca gagaaacagt
     6181 acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc ctggagaacg
     6241 cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg gaggaacagg
     6301 agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg cccccacttc
     6361 tgagacaagc aattgagctg ttcgaccggc agggagccga acctgccttc cttttcggcc
     6421 tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc gggccggccg
     6481 acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac gactttgacc
     6541 ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc cccgggtaac
     6601 tctctgcagc agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta
     6661 ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc
     6721 atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt
     6781 ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg
     6841 ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt
     6901 tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct
     6961 ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt
     7021 cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct
     7081 acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc
     7141 ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct
     7201 ccccgcctgg atcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7261 ttagccactt tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac
     7321 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     7381 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     7441 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     7501 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     7561 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     7621 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     7681 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     7741 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7801 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7861 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7921 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     7981 tcagagagtg agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg
     8041 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     8101 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     8161 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg
     8221 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
     8281 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
     8341 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
     8401 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     8461 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     8521 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     8581 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     8641 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     8701 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     8761 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     8821 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     8881 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     8941 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     9001 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     9061 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     9121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     9181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     9241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     9301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     9361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     9421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     9481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     9541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     9601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     9661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     9721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     9781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     9841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     9901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     9961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
    10021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
    10081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
    10141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
    10201 cctgacgtcg acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata
    10261 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
    10321 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa
    10381 ccaaaatcat cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt
    10441 tcatttactc taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg
    10501 ttaaatatgt actacaaact tagtagtttt taaagaaatt gtatttgtta aatatgtact
    10561 acaaacttag tagt
//
